Anti-Mouse MHC Class II (I-A) [Clone Y-3P] – Purified <i>in vivo</i> PLATINUM™ Functional Grade - 50 mg

B, FC

2 - 8°C Wet Ice

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group ofantigens divided into class I and II proteins that function in immune response1. Class IImolecules, also known as Ia antigens, regulate recognition of foreign antigens on the surfaces ofantigen presenting cells and play a major role in the mixed lymphocyte response2. Mice havetwo class II isotypes, I-A and I-E, each of which is a glycoprotein composed of an ⍺ and βsubunit. The N-terminal α1 and β1 domains of the MHC class II isotype form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens3.On APCs, MHC class II molecules play a critical role in the adaptive immune response bypresenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cellactivation leading to the coordination and regulation of other effector cells. CD4 molecules alsobind to MHC class II, which helps augment TCR signaling4. Additionally, MHC class IIexpressed on activated T cells are capable of antigen presentation5 and can transduce signals intoT cells, enhancing T cell proliferation and activity6. Y-3P was generated by repeatedly immunizing primed mice with activated T cells over thecourse of a year7. Y-3P reacts with I-A subregion-controlled A ⍺: A β complexes of all mousestrains except the responder strain H-2d. Y-3P is commonly used for in vivo blockade ofTCR stimulation8, 9 and MHC class II blocking10, 11, 12, 13, 14, 15.

≥ 5.0 mg/ml

MHC class II molecules are constitutively expressed on professionalantigen-presenting cells (APCs), including macrophages, monocytes, dendritic cells (DCs), andB cells, and are induced on T cells upon activation.