Anti-Mouse/Human PNAd (Clone MECA-79) - Purified in vivo GOLD^TM Functional Grade - 5.0 mg

2 - 8°C Wet Ice

MECA-79 antibody and L-selectin recognize the same family of sialomucins knowncollectively as peripheral node addressin (PNAd), with MECA-79 staining ligands for L-selectin expressed by high endothelial venules (HEVs)1. The MECA-79 epitopeconsists of a 6-sulfo N-acetyl-lactosamine modification of the extended core-1 branchthat overlaps with the sialyl 6-sulfo Lewis X at its terminus. Binding requires GlcNAc-6sulfation but not sialylation and/or fucosylation. MECA-79 stains lymph node HEVs andblocks L-selectin-dependent lymphocyte attachment in vitro and in vivo in mousemodels2.In vitro, MECA-79 blocks normal lymphocytes and a peripheral lymph node-specificlymphoma from binding to peripheral lymph node HEV2. In vivo, MECA-79 inhibitsnormal lymphocyte homing to peripheral lymph nodes. Additionally, MECA-79 detectsHEVs in lymph nodes of HEV-like vessels at the sites of chronic inflammation, wherePNAd + vessels are induced, and staining of these vessels corresponds to functional L-selectin ligands1. Furthermore, MECA-79 antibody treatment reduces lymphocyteadhesion to GlyCAM-1 from wildtype mouse HEV3. MECA-79 was generated by immunizing a Wistar rat with lymphocytes released frompooled axillary, brachial, inguinal, and mesenteric lymph nodes of BALB/c mice2.Spleen cells from immune animals were fused with mouse myeloma Sp2/0 to createhybridomas, which were screened by immunofluorescence for an antibody thatselectively stains peripheral lymph node HEV. MECA-79 is IgM.

≥ 5.0 mg/ml

In mice, PNAd staining is detected on all high endothelial venules(HEV) in peripheral lymph nodes with intense cytoplasmic as well as luminal andabluminal cell surface reactivity. MECA-79 stains peripheral lymph nodes, somesegments of mesenteric lymph nodes, the abluminal aspect of high endothelial cells(HEC) in Peyer’s patches, and postcapillary venules of inflamed non-lymphoid tissues.In humans, MECA-79 binds to HEC from tonsil.