AM 404

Non Antibodies

TRPV1, CB1 receptors

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

395, 6

An Activator of TRPV1 Channels and a Ligand of CB1 Cannabinoid Receptors

100 mM in DMSO, 50 mM in ethanol (dissolves slowly). Centrifuge all product preparations before use (10000 x g 5 min).

41116134

AM 404 activates TRPV1 channels with EC50 = 0.04 µM1, and serves as a ligand for CB1 cannabinoid receptors2.

Yes

N-arachidonoylaminophenol (AM 404) belongs to a group of bioactive N-acylamines. It is a potent activator of TRPV1 (EC50 = 26 nM)1, and a CB1 cannabinoid receptor ligand2. Recent work on the mechanism of AM 404 has suggested that the inhibition of fatty acid amide hydrolase (FAAH) by AM 404 (IC50 = 6 μΜ) is likely responsible for all of its attributed "reuptake" properties, since intracellular FAAH hydrolysis of anandamide changes the intra/extracellular anandamide equilibrium3. AM 404 also inhibits cyclooxygenase COX-1 and COX-2, thus attenuating prostaglandin synthesis4. AM 404 produces analgesia in the mouse formalin and hot plate tests5 and potentiates the analgesic effect of anandamide in the mouse hot plate test6. Furthermore, AM 404 decreases the expression of c-fos in the spinal cord of neuropathic rats, an effect inhibited by both TRPV1 and cannabinoid receptor antagonists7.