Cyclopiazonic Acid

ArtNr	ALO-C-750-50mg
Hersteller	Alomone
CAS-Nr.	18172-33-3
Menge	50 mg
Quantity options	10 mg 1 mg 25 mg 50 mg 5 mg 750 mg
Kategorie	Cardiovascular Research Ischemia & Reperfusion Injury Reagents & Chemicals Molecules
Typ	Small Molecules
Format	Lyophilized
Specific against	other
Purity	>99% (HPLC)
ECLASS 10.1	32160490
ECLASS 11.0	32160490
UNSPSC	12000000
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Type	Non Antibodies
Manufacturer - Category	S. Molecules
Manufacturer - Targets	SERCA pump
Country of Origin	Israel
Shipping Temperature	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Storage Conditions	Storage as Solution: Up to one week at 4°C or three months at -20°C. - Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Molecular Weight	336, 4
Manufacturer - Format	Lyophilized powder.
Short description	A Potent Inhibitor of Intracellular Ca2+-ATPase Pump
Description	CPA - A Potent Inhibitor of Intracellular Ca2+-ATPase Pump
Reconstitution	Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
PH	7, 4
UNSPSC	41116134
Origin	Penicillium griseofulvum.
Effective Concentration	1-10 µM.
Activity	Cyclopiazonic acid is a cell-permeable, reversible inhibitor of sarcoplasmic reticulum Ca2+-ATPase1, 2.
Solubility	DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Bioassay tested	Yes
Chemical Name	(6aR, 11aS, 11bR)-rel-10-Acetyl-2, 6, 6a, 7, 11a, 11b, hexahydro-7, 7-dimethyl-9H-pyrrolo[1', 2':2, 3]isoindolo[4, 5, 6-cd] indol-9-one.
Scientific Background	Cyclopiazonic acid (CPA) is a toxic fungal secondary metabolite. Chemically, it is an indole tetrameric acid. CPA was originally isolated from Penicillium cyclopium and subsequently from other P. cyclopium, Penicillium griseofulvum, Penicillium camembertii, Aspergillus flavus and Aspergillus versicolor.CPA is a highly potent, selective and reversible inhibitor of the sarcoplasmic reticulum (ER) Ca2+-ATPase pump (SERCA). This Ca2+ pump is an integral membrane protein of 994 amino acids that consists of three cytoplasmic domains containing the ATP binding site and ten transmembrane helices containing the two Ca2+ binding sites.1, 2The interchange of Ca2+ between the cell cytosol and the ER lumen by SERCA is a central process in intracellular signaling. Moreover, this process has a critical role in the muscle contraction-relaxation cycle, in which the recovery phase relies on transport of calcium into the ER by the action of the SERCA. Inhibition of this pump leads to the decrease of Ca2+ uptake from the cytosol to the ER lumen, thereby causing the depletion of Ca2+ store-operated and increase of cytosolic Ca2+.3-4CPA inhibits the Ca2+ pump by blocking the Ca2+ access channel and immobilizing a subset of transmembrane helices.5

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ALO-C-750-50mg-safety-datasheet.pdf