DL-AP5

Non Antibodies

NMDA receptors

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

197, 1

A Competitive NMDA Receptor Antagonist

Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

41116134

DL-AP5 is an NMDA receptor antagonist. IC50 = 0.28 µM (NR2A), 0.46 µM (NR2B), 1.6 µM (NR2C)1.

Yes

DL-2-Amino-5-phosphonovaleric acid (DL-AP5) is a selective NMDA receptor antagonist that competitively inhibits the glutamate binding site of NMDA receptors and blocks excitatory synaptic transmission.The NMDA receptor plays important physiological and pathological roles in the central nervous system. The receptor binds the neurotransmitter L-glutamate, and meditates the majority of the fast excitatory neurotransmission in the mammalian brain. In addition, it induces current formation in neurons1.Peripheral administration of AP5 does not cross the blood-brain-barrier and this compound may have an effect on information processing and transmission as well as plasticity2, 3.Blockade of NMDARs by AP5 has the ability to trigger synaptic potentiation in the mature hippocampus3.Evidence shows that the inhibitory effects of AP5 are independent of membrane potential but decrease with increasing stimulation frequency3.Several studies have suggested that chronic treatment of the optic tectum with DL-AP5 desegregates eye-specific stripes in three-eyed frogs4.