L-689,560

Non Antibodies

NMDA receptors

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

380, 23

A Potent and Selective Antagonist of NMDA Receptors

Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

41116134

L-689, 560 is a potent and selective antagonist at the glycine site of NMDA receptors, shown to selectively inhibit [3H]-glycine binding in rat cortex membranes with IC50 of 7.4 nM, and to inhibit NMDA-evoked currents in rat cortical neurons with plC50 of 7.461.

Yes

L-689, 560 is a potent and selective antagonist at the glycine site of N-methyl-D-aspartate (NMDA) receptors. L-689, 560 behaves as a full antagonist and competes with glycine for the receptors binding site1. It selectively inhibits [3H]-glycine binding in rat cortex membranes with an IC50 value of 7.4 nM1.NMDA receptors are heterotetrameric channels formed by the assembly of two obligatory GluN1 and two GluN2/GluN3 subunits. They play an important role in a variety of cellular processes and brain functions such as, synaptic plasticity, addiction and stroke, and mediate physiological functions such as learning and memory formation and participates in glutamate excitotoxicity2.