2-Methyl-5-hydroxytryptamine hydrochloride

Non Antibodies

5-HT3 and 5-HT6 receptors

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

226, 7

An Agonist of 5-HT3 and 5-HT6 Receptors

100 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).

41116134

Methyl serotonin is a 5-HT3 agonist (Ki = 1200 nM) and potent 5-HT6 ligand (Ki = 46 nM)1.

Yes

Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter synthesized from the amino acid tryptophan by the enzyme tryptophan-decarboxylase. The 5-HT3 channel is a ligand gated ion channel receptor of the cys-loop channel family. It is a pentamer with five subunits surrounding a central channel1.Methyl Serotonin (2-Methyl-5-hydroxytryptamine hydrochloride) is an agonist of 5-HT3 receptors with an effective concentration of 0.5-50 µM and an IC50 of 3.1±0.9 µM and also a potent ligand of the 5-HT6 receptor, member of the G-protein coupled receptor superfamily. Methyl Serotonin mimics the action of 5-HT although it displays a low affinity to 5-HT1 and 5-HT2 receptors. Methyl Serotonin was found to stimulate the hydrolysis of phosphoinositide in rat brain tissue2. In a study conducted in N1E-115 neuroblastoma cells, Methyl Serotonin elicited inward ion currents that were only 19.9±2.4% of currents elicited by regular Serotonin binding. These currents increase with higher concentration of Methyl serotonin. Desensitization of the 5-HT receptor after Methyl Serotonin binding was slower than equivalent Serotonin binding3.