Picrotoxin

ArtNr	ALO-P-325-500mg
Hersteller	Alomone
CAS-Nr.	124-87-8
Menge	500 mg
Quantity options	10 g 1 g 500 mg 5 g
Kategorie	Neuroscience Metabolism Regulation of Appetite Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Reagents & Chemicals Molecules
Typ	Small Molecules
Format	Lyophilized
Specific against	other
Purity	>99% (HPLC)
ECLASS 10.1	32160490
ECLASS 11.0	32160490
UNSPSC	12000000
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Type	Non Antibodies
Manufacturer - Category	S. Molecules
Manufacturer - Targets	GABA(A), Glycine, 5-HT3 receptors
Country of Origin	Israel
Shipping Temperature	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Storage Conditions	Storage as Solution: Up to three months at -20°C. - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Molecular Weight	602, 58
Manufacturer - Format	Lyophilized powder.
Short description	An Antagonist of GABA(A), Glycine, and 5-HT3 Receptors
Description	Cocculin, Picrotin, Picrotoxinin - An Antagonist of GABA(A), Glycine, and 5-HT3 Receptors
Reconstitution	Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water to high-micromolar concentrations (50 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
PH	7, 4
UNSPSC	41116134
Origin	Anamirta cocculus.
Effective Concentration	1-100 µM.
Activity	Picrotoxin, an equimolar mixture of picrotoxinin and picrotin, is a non-competitive antagonist of the GABA(A) receptors (IC50 = 2.4 µM - 17 µM)1, an antagonist of glycine receptors (IC50 = 2.7 µM - 6.4 µM)2 and of 5-HT3 receptors (IC50 = 41.2 µM)3.
Solubility	Soluble in DMSO and ethanol. Centrifuge before use (10000 x g, 5 min).
Bioassay tested	Yes
Chemical Name	(1R, 3R, 5S, 8S, 13R, 14S)-1-hydroxy-14-(2-hydroxypropan-2-yl)-13-methyl--6, 11-]tetradecane5, 13.03, 5.09, 12trioxapentacyclo[6.4.1.1-, 7, 104dione; (1R, 5S, 8S, 13R, 14R)-1-hydroxy-13-methyl-14-prop-1-en-2-yl-4, 7, 10-dione-6, 11-]tetradecane5, 13.03, 5.09, 12rioxapentacyclo[6.4.1.1t
Scientific Background	Picrotoxin (PTX) is a plant-derived alkaloid that originally showed to be a noncompetitive antagonist of GABA(A) receptors. Later it was discovered that picrotoxin inhibits other anion-selective ligand-gated anion channels including glycine and 5-hydroxytryptamine type 3 (5-HT3) receptors. Inhibition by picrotoxin is likely in a noncompetitive, use-facilitated manner through binding to the channel pore of the receptor1, 2.5-HT3 receptor is a member of the cys-loop superfamily of ligand-gated ion channels (LGICs) that includes nicotinic acetylcholine receptors, GABAA, glycine receptors, glutamate and purinergic receptors1, 2.

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ALO-P-325-500mg-safety-datasheet.pdf