Human beta-Actin antibody

Antibodies

Monoclonal Antibody

Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Monoclonal

Cancer

b-Actin, beta actin, beta-actin, actin, ATGA0570-10ug, ATGA0570-20ug, ATGA0570-50ug, ATGA0570-100ug, ATGA0570-250ug, ATGA0570-500ug, ATGA0570-1mg, ATGA0570-010, ATGA0570-020, ATGA0570-050, ATGA0570-100, ATGA0570-250, ATGA0570-500, ATGA0570-01M, ATGA0606-10ug, ATGA0606-20ug, ATGA0606-50ug, ATGA0606-100ug, ATGA0606-250ug, ATGA0606-500ug, ATGA0606-1mg, ATGA0606-010, ATGA0606-020, ATGA0606-050, ATGA0606-100, ATGA0606-250, ATGA0606-500, ATGA0606-01M

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: PC3 cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: U87-MG cell lysate
Lane 7.: NIH-3T3 cell lysateThe mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Kidney tissue lysate
Lane 2.: Spleen tissue lysate
Lane 3.: Bone marrow tissue lysate
Lane 4.: Stomach tissue lysate
Lane 5.: Liver tissue lysate The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: ACTB antibody 1:1000
Lane 2.: ACTB antibody 1:3000
Lane 3.: ACTB antibody 1:5000
Lane 4.: ACTB antibody 1:10000