Human 5′-Nucleotidase/CD73 antibody

Antibodies

Monoclonal Antibody

CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Monoclonal

Immunology

Purine 5-Prime-Nucleotidase, NTE, NT5E, NT5, NT, eNT, eN, ecto-5'-nucleotidase, ecto, CD73, CALJA, AI447961, 5-nucleotidase ecto, 5'-nucleotidase ecto, 5-nucleotidase, 5'-nucleotidase, 5-NT, 5'-NT, 5' nucleotidase ecto, 5 nucleotidase ecto, 5' nucleotidase, 5 nucleotidase, 5' NT, 2210401F01Rik, ACD0806-10ug, ACD0806-20ug, ACD0806-50ug, ACD0806-100ug, ACD0806-250ug, ACD0806-500ug, ACD0806-1mg, ACD0806-010, ACD0806-020, ACD0806-050, ACD0806-100, ACD0806-250, ACD0806-500, ACD0806-01M

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 5'-Nucleotidase/CD73 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: WiDr cell lysate
Lane 3.: A431 cell lysate