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Human Hexokinase 1/2 antibody

Human Hexokinase 1/2 antibody

ArtNr	NKM-AHK0812-50ul
Hersteller	NKMAX
Menge	50 ul
Quantity options	100 ul 50 ul
Kategorie	Metabolism Glucose Homeostasis Antibodies Flow Cytometry Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, FC, ICC, ELISA
Clon	1A7
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Konjugat/Tag	Unconjugated
Citations	Furuta H, et al.,(1996) Genomics 36(1):206-209. Jon E, et al., (2003) J Exp Biology 206:2049-2057.
NCBI	NP_000180
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Lieferbar
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF, FACS
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human Hexokinase 2 (1-917aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-G affinity chromatography
Gene Category	Signal Transduction
Manufacturer - Search Terms	BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase 1, Hexokinase 2, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase1, Hexokinase-1, Hexokinase2, Hexokinase-2, Hexokinase-A, HK I, HK II, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HK2, HKI, HKII, HXK 1, HXK 2, HXK1, HXK-1, HXK2, HXK-2, mHk1-s, Muscle form hexokinase, tumor isozyme, AHK0812-10ug, AHK0812-20ug, AHK0812-50ug, AHK0812-100ug, AHK0812-250ug, AHK0812-500ug, AHK0812-1mg, AHK0812-010, AHK0812-020, AHK0812-050, AHK0812-100, AHK0812-250, AHK0812-500, AHK0812-01M
Manufacturer - Alternative Names	BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase-1, Hexokinase-2, Hexokinase-A, HK I, HK II, HK1, HK1 tb, HK1-s, HK1-ta, HK1-tc, HK2, HXK1, mHk1-s, Muscle form hexokinase, Tumor isozyme
Manufacturer - Similar Names	BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase 1, Hexokinase 2, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase1, Hexokinase-1, Hexokinase2, Hexokinase-2, Hexokinase-A, HK I, HK II, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HK2, HKI, HKII, HXK 1, HXK 2, HXK1, HXK-1, HXK2, HXK-2, mHk1-s, Muscle form hexokinase, tumor isozyme, AHK0812-10ug, AHK0812-20ug, AHK0812-50ug, AHK0812-100ug, AHK0812-250ug, AHK0812-500ug, AHK0812-1mg, AHK0812-010, AHK0812-020, AHK0812-050, AHK0812-100, AHK0812-250, AHK0812-500, AHK0812-01M
IF Comment	ICC/IF analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Hexokinase 1 recombinant protein Lane 2.: Hexokinase 2 recombinant protein Lane 3.: Hexokinase 3 recombinant protein Lane 4.: Hexokinase 4 recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: K562 cell lysate Lane 2.: Jurkat cell lysate
FACS Comment	Flow cytometry analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

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