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Human HSP70/HSPA1A antibody

ArtNr NKM-AHS0703-100ul
Hersteller NKMAX
Menge 100 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, IHC, ICC, ELISA
Clon 4E7
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
Citations Lewis MJ, et al., (1985) EMBO J 4:3137-3143.
Gething M-J, et al., (1992) Nature 355:33-45.
Dick D.M, et al., (2000) Mol Cell Biol 20(19):7146-7159.
NCBI NP_005336
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Heat shock 70kDa protein 1A,Heat shock protein family A member 1A,HSP70,HSP70.1,HSP70-1/HSP70-2,HSPA1,HSPA1A,HSPA1B
Lieferbar
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF, IHC
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
Hsp70 is a human heat shock protein. Hsp70 is an important part of the cell's machinery for protein folding, and help to protect cells from stress. In most species, there are many proteins that belong to the hsp70 family. Some of these are only expressed under stress conditions, while some are present in cells under normal growth conditions and are not heat-inducible. Hsp70 can be found in different cellular compartments (nuclear, cytosolic, mitochondrial, endoplasmic reticulum, etc... ), and the chaperone function of hsp70 is required for protection against stress-induced apoptosis.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Hsp70 (1-641aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-G affinity chromatography
Gene Category
Heat Shock Proteins
Manufacturer - Search Terms
Heat shock 70 kDa protein 1A, Heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP 70, HSP70, HSP70.1, HSP70-1/HSP70-2, HSPA1, HSPA1A, HSPA1B, AHS0703-10ug, AHS0703-20ug, AHS0703-50ug, AHS0703-100ug, AHS0703-250ug, AHS0703-500ug, AHS0703-1mg, AHS0703-010, AHS0703-020, AHS0703-050, AHS0703-100, AHS0703-250, AHS0703-500, AHS0703-01M
Manufacturer - Similar Names
Heat shock 70 kDa protein 1A, Heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP 70, HSP70, HSP70.1, HSP70-1/HSP70-2, HSPA1, HSPA1A, HSPA1B, AHS0703-10ug, AHS0703-20ug, AHS0703-50ug, AHS0703-100ug, AHS0703-250ug, AHS0703-500ug, AHS0703-1mg, AHS0703-010, AHS0703-020, AHS0703-050, AHS0703-100, AHS0703-250, AHS0703-500, AHS0703-01M
IF Comment
ICC/IF analysis of HSP70 in A549 cells line. The cell was stained with AHS0703 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp70 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HeLa cell lysate
IHC Comment
Paraffin embedded sections of human eccrine gland were incubated with anti-human Hsp70 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
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