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Human KIR2DL1/KIR2DL3/KIR2DS4 antibody

ArtNr NKM-AKR0620-100ul
Hersteller NKMAX
Menge 100 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, ICC, ELISA
Clon 2F9
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG2a Kappa
Konjugat/Tag Unconjugated
Citations Shin JS, et al., 1999. Hybridoma 18:521-527.
Qing R. Fan, et al., 2001. Nature Immunology 2:452-460.
NCBI NP_055033
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Lieferbar
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF, FACS
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human Nk cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human kIR2DL1 (23-223 aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-G affinity chromatography
Gene Category
Immunology
Manufacturer - Search Terms
KIR2DL3, P43628, NP_056952.2, KIR2DS4, P43632, NP_036446, Killer cell immunoglobulin-like receptor 2DL3, CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, NKAT2a, NKAT2b, Natural killer-associated transcript 2, NKAT-2, p58 natural killer cell receptor clone CL-6, p58 NK receptor CL-6, p58.2 MHC class-I-specific NK receptor, CD158b2, KIRCL23, Killer cell immunoglobulin-like receptor 2DS4, CD158 antigen-like family member I, Natural killer-associated transcript 8, NKAT-8, P58 natural killer cell receptor clones CL-39/CL-17, p58 NK receptor CL-39/CL-17, CD158I, KKA3, KIR2DL1, CD158a, AKR0620-10ug, AKR0620-20ug, AKR0620-50ug, AKR0620-100ug, AKR0620-250ug, AKR0620-500ug, AKR0620-1mg, AKR0620-010, AKR0620-020, AKR0620-050, AKR0620-100, AKR0620-250, AKR0620-500, AKR0620-01M
Manufacturer - Alternative Names
Killer cell immunoglobulin like receptor two Ig domains and long cytoplasmic tail 1, Killer cell immunoglobulin-like receptor 2DL1, CD158 antigen-like family member A, Natural killer-associated transcript 1, NKAT-1, p58 natural killer cell receptor clones CL-42/47.11, p58 NK receptor CL-42/47.11, p58.1 MHC class-I-specific NK receptor, CD158a
Manufacturer - Similar Names
KIR2DL3, P43628, NP_056952.2, KIR2DS4, P43632, NP_036446, Killer cell immunoglobulin-like receptor 2DL3, CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, NKAT2a, NKAT2b, Natural killer-associated transcript 2, NKAT-2, p58 natural killer cell receptor clone CL-6, p58 NK receptor CL-6, p58.2 MHC class-I-specific NK receptor, CD158b2, KIRCL23, Killer cell immunoglobulin-like receptor 2DS4, CD158 antigen-like family member I, Natural killer-associated transcript 8, NKAT-8, P58 natural killer cell receptor clones CL-39/CL-17, p58 NK receptor CL-39/CL-17, CD158I, KKA3, KIR2DL1, CD158a, AKR0620-10ug, AKR0620-20ug, AKR0620-50ug, AKR0620-100ug, AKR0620-250ug, AKR0620-500ug, AKR0620-1mg, AKR0620-010, AKR0620-020, AKR0620-050, AKR0620-100, AKR0620-250, AKR0620-500, AKR0620-01M
IF Comment
ICC/IF analysis of KIR2DL1 in Hep3B cells. The cell was stained with AKR0620 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: Jurkat cell lysate The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: KIR2DL1 recombinant protein
Lane 2.: KIR2DL3 recombinant protein
Lane 3.: KIR2DS4 recombinant protein
Lane 4.: KIR2DL4 recombinant protein
FACS Comment
Flow cytometry analysis of KIR2DL1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
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