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Human Podoplanin antibody

ArtNr NKM-APD0833-100ul
Hersteller NKMAX
Menge 100 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen FC, ICC, ELISA
Clon 5E2
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG2b Kappa
Konjugat/Tag Unconjugated
Citations Wicki A., et al. (2006) Br J Cancer. 96(1):1-5.
Zimmer G., et al. (1999) Biochem J. 341(Pt2):277-84.
NCBI NP_006465
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Similar products Podoplanin, APD0833-10ug, APD0833-20ug, APD0833-50ug, APD0833-100ug, APD0833-250ug, APD0833-500ug, APD0833-1mg, APD0833-10, APD0833-20, APD0833-50, APD0833-100, APD0833-250, APD0833-500, APD0833-1
Lieferbar
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, ICC/IF, FACS
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
Podoplanin (PDPN) is a small mucin-like transmembrane protein, widely expressed in various specialized cell types throughout the body. This is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PDPN (1-206aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-G affinity chromatography
Gene Category
Cytoskeleton
Manufacturer - Search Terms
Podoplanin, APD0833-10ug, APD0833-20ug, APD0833-50ug, APD0833-100ug, APD0833-250ug, APD0833-500ug, APD0833-1mg, APD0833-10, APD0833-20, APD0833-50, APD0833-100, APD0833-250, APD0833-500, APD0833-1
Manufacturer - Alternative Names
TIA2, T1A, T1 ALPHA GENE, T1 alpha, Podoplanin, PDPN, PA2.26 antigen, OTTHuMP00000044504, OTTHuMP00000009640, OTS8, Lung type I cell membrane-associated glycoprotein isoform a, Lung type I cell membrane associated glycoprotein isoform a AGGRuS, Lung type I cell membrane associated glycoprotein, hT1alpha2, hT1alpha1, HT1A-1, GP40, Gp38, GP36, Glycoprotein 36 KD
IF Comment
ICC/IF analysis of Podoplanin in HaLe cells. The cell was stained with APD0833 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
FACS Comment
Flow cytometry analysis of Podoplanin in HeLa cells. The cell was stained with APD0833 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
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