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Human PGP9.5/UCHL1 antibody

Human PGP9.5/UCHL1 antibody

ArtNr	NKM-APG0714-50ul
Hersteller	NKMAX
Menge	50 ul
Quantity options	100 ul 50 ul
Kategorie	Neuroscience Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Antibodies Immunohistochemistry Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, IHC, ICC, ELISA
Clon	3D9
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2a Kappa
Konjugat/Tag	Unconjugated
Citations	kenji H et al , (1999) Am J Pathol 155(3):711-715. Liu Y et al , (2002) Cell 111(2):209-18. Mikako Sakurai.et al , (2006) Cell 119:162-171.
NCBI	NP_004172
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Ubiquitin C-terminal hydrolase L1,Ubiquitin carboxyl-terminal esterase L1,Ubiquitin thiolesterase L1,Parkinson Disease 5,PARK5,PGP9.5,Uch-L1,UCHL-1,Neuron cytoplasmic protein 9.5
Similar products	PGP9.5, UCH-L1, APG0714-10ug, APG0714-20ug, APG0714-50ug, APG0714-100ug, APG0714-250ug, APG0714-500ug, APG0714-1mg, APG0714-10, APG0714-20, APG0714-50, APG0714-100, APG0714-250, APG0714-500, APG0714-1
Lieferbar
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF, IHC
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	PGP9. 5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9. 5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9. 5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human PGP9.5 (1-223aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-G affinity chromatography
Gene Category	Ubiquitination
Manufacturer - Search Terms	UCH-L1, PGP9.5, APG0714-10ug, APG0714-20ug, APG0714-50ug, APG0714-100ug, APG0714-250ug, APG0714-500ug, APG0714-1mg, APG0714-10, APG0714-20, APG0714-50, APG0714-100, APG0714-250, APG0714-500, APG0714-1
IF Comment	ICC/IF analysis of PGP9.5 in U87MG cells. The cell was stained with APG0714 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse Brain tissue lysate Lane 2.: U87MG cell lysate
IHC Comment	Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 ul

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Lieferung vsl. bis 04.12.2025

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