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Human SNAP25 antibody

ArtNr NKM-ASN0720-50ul
Hersteller NKMAX
Menge 50 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, ICC, ELISA
Clon 4E11
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
Citations Schulz JR et al., (1998) J Biol Chem 273(38):24355-9.
Tafoya LC et al., (2006) J Neurosci 26(30):7826-38.
NCBI NP_570824
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Similar products SNAP25, ASN0720-10ug, ASN0720-20ug, ASN0720-50ug, ASN0720-100ug, ASN0720-250ug, ASN0720-500ug, ASN0720-1mg, ASN0720-10, ASN0720-20, ASN0720-50, ASN0720-100, ASN0720-250, ASN0720-500, ASN0720-1
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Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
The synaptosomal-associated protein (SNAP25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP25 is directly involved in the release of neurotransmitters.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human SNAP25 (1-206aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-G affinity chromatography
Gene Category
Neuroscience
Manufacturer - Search Terms
SNAP25, ASN0720-10ug, ASN0720-20ug, ASN0720-50ug, ASN0720-100ug, ASN0720-250ug, ASN0720-500ug, ASN0720-1mg, ASN0720-10, ASN0720-20, ASN0720-50, ASN0720-100, ASN0720-250, ASN0720-500, ASN0720-1
Manufacturer - Alternative Names
Synaptosomal-associated protein 25 isoform SNAP25B, SNAP25, SNAP, SNAP25, Synaptosomal-associated protein, His- tag, SNAP-25, Synaptosomal-associated 25 kDa protein, Super protein, SuP, Synaptosomal-associated protein 25, Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2, Bdr, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC9, SNAP 25, SNAP-25B, SNP 25, sp, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal Associated Protein 25, Synaptosomal associated protein 25kDa
IF Comment
ICC/IF analysis of SNAP25 in U87MG cells. The cell was stained with ASN0720 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment
The tissue lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain tissue lysate The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: SNAP25 Transfected 293T cell lysate

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ul
Lieferbar: In stock
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