Human IL-6 antibody

Antibodies

Monoclonal Antibody

Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length that plays critical roles in host defense, immune response and hematopoiesis. IL-6 produced by T cells, macrophages, fibroblasts, endothelial and other cells. IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-Beta, is important for developing Th17 responses. The IL-6 receptor is a trimeric complex composed of an IL-6-specific a chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Monoclonal

Immunology

VLAB, VLA-4 subunit bet, VLA4 subunit bet, VLA 4 subunit bet, MSK12, MSK 12, MDF2, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta1, Integrin beta 1, IL-6, IL6, IL 6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, GP IIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0123-10ug, ATGA0123-20ug, ATGA0123-50ug, ATGA0123-100ug, ATGA0123-250ug, ATGA0123-500ug, ATGA0123-1mg, ATGA0123-010, ATGA0123-020, ATGA0123-050, ATGA0123-100, ATGA0123-250, ATGA0123-500, ATGA0123-01M

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-6 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: Raw264.7 cell lysate
Lane 4.: PC3 cell lysate
Lane 5.: THP-1 cell lysate
Lane 6.: A549 cell lysate
Lane 7.: MCF7 cell lysate
Lane 8.: K562 cell lysate