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Human Retinol Binding Protein 4/RBP4 antibody

ArtNr NKM-ATGA0136-50ul
Hersteller NKMAX
Menge 50 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, ICC, ELISA
Clon AT2B4
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
Citations Tan Bk, et al., (2007) J Clin Endocrinol Metab 92(7):2590-2597.
Yao-Borengasser A, et al., (2007) J Clin Endocrinol Metab 92(7):2430-2432.
NCBI NP_006735
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Plasma retinol-binding protein,PRBP,RBP
Similar products RBP4, Retinol-Binding Protein4, ATGA0136-10ug, ATGA0136-20ug, ATGA0136-50ug, ATGA0136-100ug, ATGA0136-250ug, ATGA0136-500ug, ATGA0136-1mg, ATGA0136-10, ATGA0136-20, ATGA0136-50, ATGA0136-100, ATGA0136-250, ATGA0136-500, ATGA0136-1
Lieferbar
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF, FACS
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
Retinol binding protein 4 (RBP4) belongs to the lipocalin family and is the specific carrier for retinol in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human RBP4 (19-201aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-G affinity chromatography
Gene Category
Metabolism
Manufacturer - Search Terms
RBP4, Retinol-Binding Protein4, ATGA0136-10ug, ATGA0136-20ug, ATGA0136-50ug, ATGA0136-100ug, ATGA0136-250ug, ATGA0136-500ug, ATGA0136-1mg, ATGA0136-10, ATGA0136-20, ATGA0136-50, ATGA0136-100, ATGA0136-250, ATGA0136-500, ATGA0136-1
IF Comment
ICC/IF analysis of RBP4 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
WB Comment
The HepG2 cell lysate (60ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RBP4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RBP4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: RBP4 Transfected 293T cell lysate
FACS Comment
Flow cytometry analysis of RBP4 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ul
Lieferbar: In stock
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