Human Bcl-2 antibody

ArtNr	NKM-ATGA0190-100ul
Hersteller	NKMAX
Menge	100 ul
Quantity options	100 ul 50 ul
Kategorie	Oncology Antibodies By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Myeloma Cell Biology Cell Status Apoptosis Flow Cytometry Western Blot ELISA Immunocytochemistry Autophagy Primary Antibodies By Organ Colorectal Cancer Testicular & Prostate Cancer Immunostaining Immunochemistry
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, FC, ICC, ELISA
Clon	AT1B5
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b Kappa
Konjugat/Tag	Unconjugated
Citations	Zamzami N., et al,. (1998) Oncogene. 16(17):2265-82. Fernandez-Sarabia MJ., et al,. (1993) Nature. 366(6452):274-5.
NCBI	NP_000624
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	BCL2 apoptosis regulator,B-cell CLL/lymphoma 2,PPP1R50,protein phosphatase 1,regulatory subunit 50
Similar products	Bcl2, ATGA0190-10ug, ATGA0190-20ug, ATGA0190-50ug, ATGA0190-100ug, ATGA0190-250ug, ATGA0190-500ug, ATGA0190-1mg, ATGA0190-10, ATGA0190-20, ATGA0190-50, ATGA0190-100, ATGA0190-250, ATGA0190-500, ATGA0190-1
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Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF, FACS
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	Bcl-2 (B-cell lymphoma protein 2 alpha) is an anti-apoptotic protein located primarily in the outer mitochondrial membrane. It blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apoptosis. Bcl-2 protein has anti-apoptotic functions through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human Bcl-2 (1-211aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-G affinity chromatography
Gene Category	Cancer
Manufacturer - Search Terms	Bcl2, ATGA0190-10ug, ATGA0190-20ug, ATGA0190-50ug, ATGA0190-100ug, ATGA0190-250ug, ATGA0190-500ug, ATGA0190-1mg, ATGA0190-10, ATGA0190-20, ATGA0190-50, ATGA0190-100, ATGA0190-250, ATGA0190-500, ATGA0190-1
IF Comment	Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with monoclonal anti-human Bcl-2 antibody (1:500) with Texas Red (Red).
WB Comment	The cell lysate of NIH3T3 (35ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Bcl-2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
FACS Comment	Flow cytometry analysis of Bcl-2 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

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Menge: 100 ul

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Lieferung vsl. bis 04.12.2025

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