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Human Cytokeratin 19 antibody

ArtNr NKM-ATGA0255-50ul
Hersteller NKMAX
Menge 50 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, ICC, ELISA
Clon AT13D10
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG2b Kappa
Konjugat/Tag Unconjugated
Citations Stone, M.R., et al. (2005) Mol Biol Cell 16: 4280-4293.
Lacroix, M. (2006) Endocrine Related Cancer 13(4): 1033-1067.
Allard W.J., et al. (2004) Clin Cancer Research 10(20): 6897-6904.
NCBI NP_002267
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Keratin type I cytoskeletal 19,Keratin,type I cytoskeletal 19,CK19,K19,K1CS
Similar products Cytokeratin19, ATGA0255-10ug, ATGA0255-20ug, ATGA0255-50ug, ATGA0255-100ug, ATGA0255-250ug, ATGA0255-500ug, ATGA0255-1mg, ATGA0255-10, ATGA0255-20, ATGA0255-50, ATGA0255-100, ATGA0255-250, ATGA0255-500, ATGA0255-1
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Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF, FACS
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
KRT19 (type 1 cytokeratin 19) is 44kDa intermediate filament (IF) protein that is part of the Keratin family of proteins. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human KRT19 (1-400aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-A affinity chromatography
Gene Category
Cytoskeleton
Manufacturer - Search Terms
Cytokeratin19, ATGA0255-10ug, ATGA0255-20ug, ATGA0255-50ug, ATGA0255-100ug, ATGA0255-250ug, ATGA0255-500ug, ATGA0255-1mg, ATGA0255-10, ATGA0255-20, ATGA0255-50, ATGA0255-100, ATGA0255-250, ATGA0255-500, ATGA0255-1
IF Comment
ICC/IF analysis of KRT19 in HepG2 cells. The cell was stained with ATGA0255 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment
The cell lysate of MCF7 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysate (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: KRT19 Transfected 293T cell lysate
FACS Comment
Flow cytometry analysis of KRT19 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ul
Lieferbar: In stock
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