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Human Cathepsin S antibody

Human Cathepsin S antibody

ArtNr	NKM-ATGA0264-50ul
Hersteller	NKMAX
Menge	50 ul
Quantity options	100 ul 50 ul
Kategorie	Oncology Antibodies Cell Biology Cell Status Apoptosis Western Blot ELISA Immunocytochemistry Primary Antibodies By Organ Immunostaining Uterine, Ovarian & Cervical Cancer Immunochemistry
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, ICC, ELISA
Clon	AT1F9
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b Kappa
Konjugat/Tag	Unconjugated
Citations	Shi. G.P., et al. (1994) J Biol Chem 269: 11530-11536. Lemere. C.A., et al. (1995) Am J Pathol 146(4): 848-860. Shi. G.P., et al. (1992) J Biol Chem 267(11): 7258-7262.
NCBI	NP_004070
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Cathepsin S,MGC3886
Similar products	CTSS, Cathepsin S, ATGA0264-10ug, ATGA0264-20ug, ATGA0264-50ug, ATGA0264-100ug, ATGA0264-250ug, ATGA0264-500ug, ATGA0264-1mg, ATGA0264-10, ATGA0264-20, ATGA0264-50, ATGA0264-100, ATGA0264-250, ATGA0264-500, ATGA0264-1
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Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	CTSS (Cathepsin S) is a lysosomal enzyme that belongs to the papain family of cysteine proteases. This protein is expressed by antigen presenting cells including macrophages, B-lymphocytes, dendritic cells and microglia. Moreover, CTSS is expressed in some epithelial cells. In contrast, cortical thymic epithelial cells do not express CTSS. Normal epidermal keratinocytes do not express CTSS. However, level of CTSS is elevated in psoriatic keratinocytes due to stimulation by proinflammatory factors. CTSS can function as an elastase over a broad pH range in alveolar macrophages.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human CTSS (17-331aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Immunology
Manufacturer - Search Terms	Cathepsin S, CTSS, ATGA0264-10ug, ATGA0264-20ug, ATGA0264-50ug, ATGA0264-100ug, ATGA0264-250ug, ATGA0264-500ug, ATGA0264-1mg, ATGA0264-10, ATGA0264-20, ATGA0264-50, ATGA0264-100, ATGA0264-250, ATGA0264-500, ATGA0264-1
IF Comment	ICC/IF analysis of CTSS in U87MG cells. The cell was stained with ATGA0264 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of CTSS in HeLa cells. The cell was stained with ATGA0264 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0264-IF2.jpg}ICC/IF analysis of CTSS in A549 cells. The cell was stained with ATGA0264 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0264-IF3.jpg}
WB Comment	The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human CTSB Lane 2.: Recombinant Human CTSD Lane 3.: Recombinant Human CTSE Lane 4.: Recombinant Human CTSF Lane 5.: Recombinant Human CTSH Lane 6.: Recombinant Human CTSK Lane 7.: Recombinant Human CTSL Lane 8.: Recombinant Human CTSS Lane 9.: Recombinant Human CTSW Lane 10.: Recombinant Human CTSZ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: U87MG cell lysate Lane 3.: A549 cell lysate

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