Human IGF-1 antibody

ArtNr	NKM-ATGA0311-100ul
Hersteller	NKMAX
Menge	100 ul
Quantity options	100 ul 50 ul
Kategorie	Oncology Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Antibodies By Cell Type Sarcoma Cell Biology Cell Culture Cytokines Western Blot ELISA Immunocytochemistry Endocrine Regulation of Lipid Metabolism Primary Antibodies Immunostaining Immunochemistry
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, ICC, ELISA
Clon	AT6F8
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b Kappa
Konjugat/Tag	Unconjugated
Citations	Hoppener J.W., et al. (1985) Hum Genet 69(2): 157-160 Jansen M., et al. (1983) Nature 306(5943): 609-611 Salmon W., et al. (1957) J Lab Clin Med 49(6): 825-836 Keating G.M., (2008) BioDrugs 22(3): 177-188 Rinderknecht E., et al. (1978) J Biol Chem 253(8): 2769-2776
NCBI	NP_000609
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	IBP1,IGF-1,IGFI,IGF-IA,IGF-IB,Insulin like growth factor 1 (somatomedin C),Insulin like growth factor IA,Insulin like growth factor IB,Insulin-like growth factor-1 IGF IA,Mechano growth factor,MGF,Somatomedin C
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Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. IGF-1 has also been referred to as a Sulfation factor and its effects were termed nonsuppressible insulin-like activity (NSILA) in the 1970s. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin, is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7, 649 daltons.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human IGF1 (49-118aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Hormones & Cytokines
Manufacturer - Search Terms	IBP1, IGF-IB, IGF IB, IGF1, IGF-1, IGFI, IGF-I, IGFIA, IGF-IA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor-1, Insulin-like growth factor-1 IGF IA, Mechano growth factor, MGF, Somatomedin C, Somatomedin-C, ATGA0311-10ug, ATGA0311-20ug, ATGA0311-50ug, ATGA0311-100ug, ATGA0311-250ug, ATGA0311-500ug, ATGA0311-1mg, ATGA0311-010, ATGA0311-020, ATGA0311-050, ATGA0311-100, ATGA0311-250, ATGA0311-500, ATGA0311-01M
Manufacturer - Similar Names	IBP1, IGF-IB, IGF IB, IGF1, IGF-1, IGFI, IGF-I, IGFIA, IGF-IA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor-1, Insulin-like growth factor-1 IGF IA, Mechano growth factor, MGF, Somatomedin C, Somatomedin-C, ATGA0311-10ug, ATGA0311-20ug, ATGA0311-50ug, ATGA0311-100ug, ATGA0311-250ug, ATGA0311-500ug, ATGA0311-1mg, ATGA0311-010, ATGA0311-020, ATGA0311-050, ATGA0311-100, ATGA0311-250, ATGA0311-500, ATGA0311-01M
IF Comment	ICC/IF analysis of IGF1 in PC3 cells. The cell was stained with ATGA0311 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The human IGF1 recombinant protein (12.5ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: IGF1 transfected 293T cell lysate

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Menge: 100 ul

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