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Human Keratin 14/KRT14 antibody

Human Keratin 14/KRT14 antibody

ArtNr	NKM-ATGA0338-50ul
Hersteller	NKMAX
Menge	50 ul
Quantity options	100 ul 50 ul
Kategorie	Antibodies Cell Biology Cytoskeleton Flow Cytometry Immunohistochemistry Western Blot ELISA Immunocytochemistry Primary Antibodies Intermediate filaments Immunostaining Immunochemistry
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, FC, IHC, ICC, ELISA
Clon	AT13F4
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2a Kappa
Konjugat/Tag	Unconjugated
Citations	Pfendner E.G., et al. (2005) J Invest Dermatol. 125(2): 239-243. Arin M.J., et al (2010) Br J Dermatol. 162(6): 1365-1369. Muller F.B., et al (2006) Hum Mutat. 27(7): 719-720.
NCBI	AAH19097
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Keratin 14,cytokeratin 14,GIG36,MGLAP,NTI
Similar products	Cytokeratin14, ATGA0338-10ug, ATGA0338-20ug, ATGA0338-50ug, ATGA0338-100ug, ATGA0338-250ug, ATGA0338-500ug, ATGA0338-1mg, ATGA0338-10, ATGA0338-20, ATGA0338-50, ATGA0338-100, ATGA0338-250, ATGA0338-500, ATGA0338-1
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Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF, IHC, FACS
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	Keratin 14 (KRT14) is a member of the type I keratin family of intermediate filament proteins. KRT14 gene provides instructions for making a protein called keratin 14. Keratins are a group of tough, fibrous proteins that form the structural framework of certain cells, particularly cells that make up the skin, hair, and nails. Keratin 14 is specifically produced in cells called keratinocytes in the outer layer of the skin.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human KRT14 (1-472aa) purified from E. coli.
Ab Usage	The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Cytoskeleton
Manufacturer - Search Terms	Cytokeratin14, ATGA0338-10ug, ATGA0338-20ug, ATGA0338-50ug, ATGA0338-100ug, ATGA0338-250ug, ATGA0338-500ug, ATGA0338-1mg, ATGA0338-10, ATGA0338-20, ATGA0338-50, ATGA0338-100, ATGA0338-250, ATGA0338-500, ATGA0338-1
IF Comment	ICC/IF analysis of KRT14 in PC3 cells. The cell was stained with ATGA0338 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The recombinant protein (50ng) and cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human KRT14 Lane 2.: A431 cell lysate
FACS Comment	Flow cytometry analysis of KRT14 in A431 cells. The cell was stained with ATGA0338 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
IHC Comment	Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT14 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Lieferung vsl. bis 04.12.2025

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