Human Fumarase/FH antibody

Antibodies

Monoclonal Antibody

Fumarase (Fumarate hytdratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid (TCA), or Krebs cycle. This enzyme exists in both a cytosolic form and an N-terminal extended mitochondrial form. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension is the same form as in the cytoplasm. Fumarase deficiency can lead to progressive encephalopathy, cerebral atrophy and developmental delay and this enzyme also is thought to act as a tumor suppressor.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Monoclonal

Cell Cycle

Fumarase, Fumarate hydratase, FH, HLRCC, LRCC, MCL, MCuL1, Fumarate hydratase Fumarase, Fumarate hydratase mitochondrial, MCuL 1, Multiple hereditary cutaneous leiomyomata., ATGA0366-10ug, ATGA0366-20ug, ATGA0366-50ug, ATGA0366-100ug, ATGA0366-250ug, ATGA0366-500ug, ATGA0366-1mg, ATGA0366-10, ATGA0366-20, ATGA0366-50, ATGA0366-100, ATGA0366-250, ATGA0366-500, ATGA0366-1

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Fumarase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : LNCap cell lysate
Lane 3. : A549 cell lysate
Lane 4. : HepG2 cell lysate
Lane 5. : 293T cell lysate
Lane 6. : K562 cell lysate
Lane 7. : Jurkat cell lysate