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Human S100A11 antibody

ArtNr NKM-ATGA0434-100ul
Hersteller NKMAX
Menge 100 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, ICC, ELISA
Clon AT20D11
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
Citations Sakaguchi M., et al. (2003) J Cell Biol. 163: 825-35.
Settmacher U., et al. (2005) Int. J. Oncol. 28: 195-200.
NCBI NP_005611
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias S100 calcium binding protein A11,MLN70,S100C
Similar products S100A11, MLN70, S100C, S100 calcium binding protein A11, ATGA0434-10ug, ATGA0434-20ug, ATGA0434-50ug, ATGA0434-100ug, ATGA0434-250ug, ATGA0434-500ug, ATGA0434-1mg, ATGA0434-10, ATGA0434-20, ATGA0434-50, ATGA0434-100, ATGA0434-250, ATGA0434-500, ATGA0434-1
Lieferbar
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF, FACS
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
S100A11 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human S100A11 (1-105aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-A affinity chromatography
Gene Category
Cell Cycle
Manufacturer - Search Terms
S100A11, S100 calcium binding protein A11, MLN70, S100C, ATGA0434-10ug, ATGA0434-20ug, ATGA0434-50ug, ATGA0434-100ug, ATGA0434-250ug, ATGA0434-500ug, ATGA0434-1mg, ATGA0434-10, ATGA0434-20, ATGA0434-50, ATGA0434-100, ATGA0434-250, ATGA0434-500, ATGA0434-1
IF Comment
ICC/IF analysis of S100A11 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
WB Comment
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : MCF7 cell lysate
Lane 3. : PC3 cell lysate
Lane 4. : THP1 cell lysate
Lane 5. : U87MG cell lysate
Lane 6. : A427 cell lysate
Lane 7. : SK-OV-3 cell lysate
Lane 8. : A549 cell lysate
FACS Comment
Flow cytometry analysis of S100a11 in HeLa cells. The cell was stained with ATGA0434 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). Flow cytometry analysis of S100a11 in A549 cells. The cell was stained with ATGA0434 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). {2004010373111810.jpg}

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
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