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Human EphA2 antibody

ArtNr NKM-ATGA0441-50ul
Hersteller NKMAX
Menge 50 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, ICC, ELISA
Clon AT66G9
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG2b Kappa
Konjugat/Tag Unconjugated
Citations Flanagan JG., et al. (1998) Annu Rev Neurosci. 21: 309-345.
Cheng N., et al. (2002) Cytokine Growth Factor Rev. 13: 75-85.
NCBI NP_004422
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias EPH receptor A2,Ephrin type-A receptor 2,EPHA2,Epithelial cell kinase,Tyrosine-protein kinase receptor ECK,ARCC2,CTPA,CTPP1,CTRCT6
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Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
EPHA2, also known as ephrin type-A receptor 2, is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. It is implicated as positional labels that may guide the development of neural topographic maps. It has also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human EPHA2 (27-537aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot analysis, and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-A affinity chromatography
Gene Category
Signal Transduction
Manufacturer - Search Terms
EphA2, Ephrin type-A receptor 2, EPHA2, ARCC2, CTPA, CTPP1, CTRCT6, ECK, AW545284, EC 2.7.10.1, Eck , Eph receptor A2, EPHA2, EPHA2_HUMAN, Ephrin receptor, Ephrin receptor EphA2, Ephrin type A receptor 2, Ephrin type-A receptor 2, Epithelial cell kinase, Epithelial cell receptor protein tyrosine kinase, Myk 2, Myk2, Sek 2Sek2, Tyrosine protein kinase receptor ECK, Tyrosine-protein kinase receptor ECK, Tyrosine-protein kinase receptor MPK-5, Tyrosine-protein kinase receptor SEK-2, ATGA0441-10ug, ATGA0441-20ug, ATGA0441-50ug, ATGA0441-100ug, ATGA0441-250ug, ATGA0441-500ug, ATGA0441-1mg, ATGA0441-10, ATGA0441-20, ATGA0441-50, ATGA0441-100, ATGA0441-250, ATGA0441-500, ATGA0441-1
Manufacturer - Similar Names
EphA2, Ephrin type-A receptor 2, EPHA2, ARCC2, CTPA, CTPP1, CTRCT6, ECK, AW545284, EC 2.7.10.1, Eck , Eph receptor A2, EPHA2, EPHA2_HUMAN, Ephrin receptor, Ephrin receptor EphA2, Ephrin type A receptor 2, Ephrin type-A receptor 2, Epithelial cell kinase, Epithelial cell receptor protein tyrosine kinase, Myk 2, Myk2, Sek 2Sek2, Tyrosine protein kinase receptor ECK, Tyrosine-protein kinase receptor ECK, Tyrosine-protein kinase receptor MPK-5, Tyrosine-protein kinase receptor SEK-2, ATGA0441-10ug, ATGA0441-20ug, ATGA0441-50ug, ATGA0441-100ug, ATGA0441-250ug, ATGA0441-500ug, ATGA0441-1mg, ATGA0441-10, ATGA0441-20, ATGA0441-50, ATGA0441-100, ATGA0441-250, ATGA0441-500, ATGA0441-1
IF Comment
ICC/IF analysis of EPHA2 in HeLa cell line, stained monoclonal anti-human EPHA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of EPHA2 in A431 cell line, stained monoclonal anti-human EPHA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0441-IF2.jpg}
WB Comment
The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : PC3 cell lysate Recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Recombinant human EPHA2 extracellular domain
Lane 2 : Recombinant human EPHA2 cytoplasmic domain

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ul
Lieferbar: In stock
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