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Human Integrin beta 1/CD29 antibody

ArtNr NKM-ATGA0485-100ul
Hersteller NKMAX
Menge 100 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, ICC, ELISA
Clon AT47E2
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
Citations Pellinen T., et al. (2008) Dev. Cell. 15: 371-385.
Chuang N.N., et al. (2007) Biochem. Soc. Trans. 35:1292-1294.
NCBI NP_002202
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Integrin beta 1,Integrin beta 1,CD29,FNRB,MDF2,VLAB,GPIIA,MSK12,Fibronectin receptor subunit beta,Glycoprotein Iia,GPIIA,VLA-4 subunit bet
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Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human ITGB1 (21-461aa) purified from E. coli.
Ab Usage
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-A affinity chromatography
Gene Category
Neuroscience
Manufacturer - Search Terms
VLA-BETA, VLAB, VLA-4 subunit beta, NRK2, Nicotinamide riboside kinase 2, MSK12, MIBP, MDF2, ITGB1, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta-1 isoform 1A, Integrin beta 1, Integrin b1, Il-6, IL6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0485-10ug, ATGA0485-20ug, ATGA0485-50ug, ATGA0485-100ug, ATGA0485-250ug, ATGA0485-500ug, ATGA0485-1mg, ATGA0485-010, ATGA0485-020, ATGA0485-050, ATGA0485-100, ATGA0485-250, ATGA0485-500, ATGA0485-01M
Manufacturer - Similar Names
VLA-BETA, VLAB, VLA-4 subunit beta, NRK2, Nicotinamide riboside kinase 2, MSK12, MIBP, MDF2, ITGB1, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta-1 isoform 1A, Integrin beta 1, Integrin b1, Il-6, IL6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0485-10ug, ATGA0485-20ug, ATGA0485-50ug, ATGA0485-100ug, ATGA0485-250ug, ATGA0485-500ug, ATGA0485-1mg, ATGA0485-010, ATGA0485-020, ATGA0485-050, ATGA0485-100, ATGA0485-250, ATGA0485-500, ATGA0485-01M
IF Comment
ICC/IF analysis of ITGB1 in HeLa cells. The cell was stained with ATGA0485 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ATGA0485-IF1b.jpg}
WB Comment
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ITGB1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: A549 cell lysate
Lane 2.: PC3 cell lysate
Lane 3.: HT1080 cell lysate

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
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