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Human alpha-Fetoprotein/AFP antibody

Human alpha-Fetoprotein/AFP antibody

ArtNr	NKM-ATGA0488-100ul
Hersteller	NKMAX
Menge	100 ul
Quantity options	100 ul 50 ul
Kategorie	Oncology Neuroscience Targets for Rodent Neurosciences Antibodies By Cell Type Carcinoma Flow Cytometry Western Blot ELISA Immunocytochemistry Primary Antibodies By Organ Lung Cancer Hepatic Cancer Immunostaining Immunochemistry
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, FC, ICC, ELISA
Clon	AT73E10
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Konjugat/Tag	Unconjugated
Citations	Tomasi TB Jr., (1997) Annu Rev Med. 28: 453-465. Elbrecht A, et al. (1992) Science. 255: 467-470.
NCBI	NP_001125
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Alpha-fetoprotein,AFPD,FETA,HPAFP
Similar products	AFP, a-Fetoprotein, ATGA0488-10ug, ATGA0488-20ug, ATGA0488-50ug, ATGA0488-100ug, ATGA0488-250ug, ATGA0488-500ug, ATGA0488-1mg, ATGA0488-10, ATGA0488-20, ATGA0488-50, ATGA0488-100, ATGA0488-250, ATGA0488-500, ATGA0488-1
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Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF, FACS
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	AFP, also known as alpha-fetoprotein, is a major plasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. This protein binds to copper, nickel, fatty acids and bilirubin. The main function of this is to prevent the virilization of female fetuses. As it does not bind estrogen, its function in humans is less clear.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human AFP (19-609aa) purified from insect cell.
Ab Usage	The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Cardiovascular
Manufacturer - Search Terms	a-Fetoprotein, AFP, ATGA0488-10ug, ATGA0488-20ug, ATGA0488-50ug, ATGA0488-100ug, ATGA0488-250ug, ATGA0488-500ug, ATGA0488-1mg, ATGA0488-10, ATGA0488-20, ATGA0488-50, ATGA0488-100, ATGA0488-250, ATGA0488-500, ATGA0488-1
IF Comment	ICC/IF analysis of AFP in HaLe cells. The cell was stained with ATGA0488 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AFP antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: Mouse liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AFP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: Raw264.7 cell lysate
FACS Comment	Flow cytometry analysis of AFP in HeLa cells. The cell was stained with ATGA0488 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ul

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