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Human Glutathione S-transferase pi 1/GSTP1 antibody

Human Glutathione S-transferase pi 1/GSTP1 antibody

ArtNr	NKM-ATGA0498-100ul
Hersteller	NKMAX
Menge	100 ul
Quantity options	100 ul 50 ul
Kategorie	Neuroscience Molecular Targets for Neuroscience Antibodies Flow Cytometry Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, FC, ICC, ELISA
Clon	AT12C10
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Konjugat/Tag	Unconjugated
Citations	Lee KA, et al. (2001) Blood. 98(12): 3483-5. Hayes JD,et al. (1995) Crit Rev BiochemMol Biol. 30(6): 445-600.
NCBI	NP_000843
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Glutathione S-transferase P,DFN7,FAEES3,GST3,PI,Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III,Glutathione S Transferase Pi,GSTP1,Glutathione S-Transferase pi 1
Similar products	GSTP1, Glutathione S-Transferase pi1, ATGA0498-10ug, ATGA0498-20ug, ATGA0498-50ug, ATGA0498-100ug, ATGA0498-250ug, ATGA0498-500ug, ATGA0498-1mg, ATGA0498-10, ATGA0498-20, ATGA0498-50, ATGA0498-100, ATGA0498-250, ATGA0498-500, ATGA0498-1
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Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF, FACS
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	GSTP1 is a glutathione S-transferase that belongs to the pi class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human GSTP1 (1-210aa) purified from E.coli.
Ab Usage	The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Apoptosis
Manufacturer - Search Terms	Glutathione S-Transferase pi1, GSTP1, ATGA0498-10ug, ATGA0498-20ug, ATGA0498-50ug, ATGA0498-100ug, ATGA0498-250ug, ATGA0498-500ug, ATGA0498-1mg, ATGA0498-10, ATGA0498-20, ATGA0498-50, ATGA0498-100, ATGA0498-250, ATGA0498-500, ATGA0498-1
IF Comment	ICC/IF analysis of GSTP1 in A549 cells. The cell was stained with ATGA0498 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: K562 cell lysate Lane 2.: Jurkat cell lysate Lane 3.: HeLa cell lysate Lane 4.: 293T cell lysate Lane 5.: A549 cell lysate
FACS Comment	Flow cytometry analysis of GSTP1 in K562 cell line. The cell was stained with ATGA0498 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ul

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Lieferung vsl. bis 04.12.2025

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