Recombinant human CuL1 protein

Proteins

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Cullin1, also known CuL1, is a core component of multiple cullin-RING-based SCF (SKP1-CuL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, it serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cullin1 may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Recombinant human CuL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl

Cell Cycle

Human