Recombinant human CKMT1A protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Recombinant human CKMT1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Metabolism

Specific activity is > 50unit/mg, and is defined as the amount of enzyme that convert 1.0 umole of phosphate from phosphocreatine to ADP per minute at pH 7.5 at 37C.

CKMT1A, Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK, ATGP1590-10ug, ATGP1590-20ug, ATGP1590-50ug, ATGP1590-100ug, ATGP1590-250ug, ATGP1590-500ug, ATGP1590-1mg, ATGP1590-10, ATGP1590-20, ATGP1590-50, ATGP1590-100, ATGP1590-250, ATGP1590-500, ATGP1590-1