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Recombinant human IL-12 p70 protein

ArtNr NKM-ATGP2843-250ug
Hersteller NKMAX
Menge 250 ug
Quantity options 10 ug 250 ug 50 ug
Kategorie
Typ Proteins Recombinant
Format Liquid
Applikationen SDS-PAGE
Specific against Human (Homo sapiens)
Host Baculovirus-Infected Insect Cells
Konjugat/Tag HIS
Purity > 90% by SDS-PAGE
Sequence IL12B(p40);;IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ L
Citations Barton J.L., et al. (2000) Eur. J. Immunol. 30:3299-3308
Bazan J.F., et al. (2001) J. Immunol. 167:1440-1446
Kim HR, et al. Anti-cancer activity and mechanistic features of a NK cell activating molecule. (Cancer Immunol Immunother. 2009){https://pubmed.ncbi.nlm.nih.gov/19259669/}
NCBI NP_002178(p40)AAK84425(p35)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias IL12A,IL12B,CLMF p35,CLMF p40,Cytotoxic lymphocyte maturation factor 35 kDa subunit,Cytotoxic lymphocyte maturation factor 40 kDa subunit,Interleukin 12 alpha chain,Interleukin-12 beta chain,NK cell stimulatory factor chain 1,p35,NK cell stimulatory factor chain 2,p40,NKSF1,NKSF2
Similar products IL12, ATGP2843-10ug, ATGP2843-20ug, ATGP2843-50ug, ATGP2843-100ug, ATGP2843-250ug, ATGP2843-500ug, ATGP2843-1mg, ATGP2843-10, ATGP2843-20, ATGP2843-50, ATGP2843-100, ATGP2843-250, ATGP2843-500, ATGP2843-1
Lieferbar
Manufacturer - Type
Recombinant Protein
Manufacturer - Applications
SDS-PAGE, Bioactivity
Manufacturer - Host
Baculovirus
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight
34.6 kDa (p40, 306aa); ; 23.3 kDa (p35, 203aa) ; ; The heterodimeric IL12 has a predicted Molecular weight of 57.9 kDa (509aa). Molecular weight on SDS-PAGE will appear higher.
Description
IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
Concentration
0.5mg/ml (determined by absorbance at 280nm)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Antigen Species
Human
Gene Category
Hormones & Cytokines
Domain
23-328aa(p40)/23-219aa(p35)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment Only
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤1ng/ml.
Predicted nterm
Met1
Species of Protein
Human
Manufacturer - Search Terms
IL12, ATGP2843-10ug, ATGP2843-20ug, ATGP2843-50ug, ATGP2843-100ug, ATGP2843-250ug, ATGP2843-500ug, ATGP2843-1mg, ATGP2843-10, ATGP2843-20, ATGP2843-50, ATGP2843-100, ATGP2843-250, ATGP2843-500, ATGP2843-1
Manufacturer - Alternative Names
IL12A, IL12B, CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Interleukin 12 alpha chain, Interleukin-12 beta chain, NK cell stimulatory factor chain 1, p35, NK cell stimulatory factor chain 2, p40, NKSF1, NKSF2

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 250 ug
Lieferbar: In stock
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