Recombinant human Akt1 protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Recombinant human AKT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Liquid in. Phosphate-Buffered Saline (pH 7.4) 20% glycerol

Signal Transduction

< 1 EU per 1ug of protein (determined by LAL method)

Akt1, ATGP3118-10ug, ATGP3118-20ug, ATGP3118-50ug, ATGP3118-100ug, ATGP3118-250ug, ATGP3118-500ug, ATGP3118-1mg, ATGP3118-10, ATGP3118-20, ATGP3118-50, ATGP3118-100, ATGP3118-250, ATGP3118-500, ATGP3118-1