Recombinant mouse PKM protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Pkm, also known as pyruvate kinase PKM isoform M2, is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. Pkm interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant mouse Pkm, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH8.5) containing 0.2M NaCl, 1mM DTT, 30% glycerol

Cancer

Specific activity: > 50, 000pmol/min/ug. One unit will convert 1.0pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C

Pkm, Pyruvate kinase PKM isoform M2, AA414905, AL024370, AL024424, Pk-2, Pk-3, Pk3, Pkm2, ATGP3610-10ug, ATGP3610-20ug, ATGP3610-50ug, ATGP3610-100ug, ATGP3610-250ug, ATGP3610-500ug, ATGP3610-1mg, ATGP3610-10, ATGP3610-20, ATGP3610-50, ATGP3610-100, ATGP3610-250, ATGP3610-500, ATGP3610-1