Recombinant human NFATC2 protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

NFATC2, also known as nuclear factor of activated T-cells cytoplasmic 2 isoform C, is a member of the nuclear factor of activated T cells family. This protein plays a vital role in the course of T helper cell activation, differentiation, and effector function. Although KO of an individual NFAT isoform in T cells leads to rather mild effects, T cells deficient for NFATC1 and 2 completely fail to produce T helper cell effector cytokines, such as the interleukins IL-2 and IL-4 or interferon gamma. Also, it is highly phosphorylated and retained in the cytoplasm. Following T cell receptor (TCR) stimulation, dephosphorylation by calcium-activated calcineurin induces a conformational change of NFATC2 that exposes one or more nuclear localization sequences. Recombinant human NFATC2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol, 1mM DTT

Signal Transduction

< 1 EU per 1ug of protein (determined by LAL method)

Human