Recombinant mouse Carboxyl Ester Lipase/CEL protein

Mouse

Specific activity is > 100, 000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C

CEL, also known as bile salt-activated lipase, is one of the Type-B carboxyl esterase/lipase family of enzymes. It is an enzyme produced by the adult pancreas and aids in the digestion of fats. Also, it catalyzes fat and vitamin absorption and acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides. This protein is highly expressed by pancreatic acinar cells and secreted into the gastrointestinal tract. Recombinant mouse CEL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Xiao X., et al. (2016) J Biol Chem. 291:23224-23236.
Shindo K., et al. (2017) Oncotarget. 8:50824-50831.

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.