ichorRed Nucleic Acid Gel Stain (10,000x in DMSO)

ichorRed Nucleic Acid Gel Stain is the third generation non-mutagenic fluorescent nucleic acid gel stain to replace the highly toxic traditional ethidium bromide (EB) or the expensive GelRed (GR) from Biotium for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. ichorRed is more sensitive than EB and GR either as precast gel stains or post gel stains. We can directly replace EB or GR with ichorRed without changing the existing imaging system since they all have the similar spectra. ichorRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. Gel staining with ichorRed is compatible with downstream applications such as gel extraction and cloning. ichorRed is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

Both ichorRed at 3x concentration is classifed as nonhazardous to aquatic life, under CCR Title 22 regulation. ichorRed at 3x or lower concentrations can be safely released into the environment.

Dilute the ichorRed 10, 000X stock reagentca.3, 300 fold to make a 3X staining solution in H2O. Note: including 0.1M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused.Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 3X staining solution to submerge the gel. Agitate the gel gently at room temperature for 30 minutes. ichorRed: Image the stained gel with a standard transilluminator (302 or 312nm), and photograph the gel using an ethidium bromide filterStaining solution can be resued 2-3 times. Store staining solution at room temperature protected from light.

Room temperature protected from light for at least one year