Vergleich

ichorGreen Nucleic Acid Gel Stain (10,000x in water) Europäischer Partner

Hersteller IchorBio
Kategorie
Typ Nucleic acid label
Specific against other
Menge 5ml
ArtNr ICH9003-5ML
eClass 6.1 32160414
eClass 9.0 32160414
Lieferbar
Background
ichorGreen Nucleic Acid Gel Stain is the third generation non-mutagenic fluorescent nucleic acid gel stain to replace the SYBR Green (SG) or the expensive GelGreen (GG) from Biotium for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. ichorGreen is more sensitive than SG and GR either as precast gel stains or post gel stains. We can directly replace SG or GG with ichorGreen without changing the existing imaging system since they all have the similar spectra. ichorGreen can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. Gel staining with ichorGreen is compatible with downstream applications such as gel extraction and cloning. ichorGreen is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
AMES Test
ichorGreen was negative in the Ames Test using Salmonella strain TA97, TA98, TA100 and TA102 (with/without S9 metabolic activation), as the revertant colonies number is less than two times of the initiative revertant colonies number with/without S9 metabolic activation. The same data was obtained by the repeated experiment
Aquatic Toxicity Test
ichorGreen at 3x concentration is classified as nonhazardous to aquatic life, under CCR Title 22 regulation. ichorGreen at 3x or lower concentrations can be safely released into the environment.
Features
Safety: Nonmutagenic and noncytotoxic Easy disposal: Safe to dispose in the drain Compatibility: Spectrally compatible with existing instruments Sensitivity: Higher signal but lower background Stability: can be stored at RT and microwavable
Post-staining agarose or acrylamide gels
Dilute the ichorRed 10, 000X stock reagentca.3, 300 fold to make a 3X staining solution in H2O. Note: including 0.1M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused.Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 3X staining solution to submerge the gel. Agitate the gel gently at room temperature for 30 minutes. ichorRed: Image the stained gel with a standard transilluminator (302 or 312nm), and photograph the gel using an ethidium bromide filterStaining solution can be resued 2-3 times. Store staining solution at room temperature protected from light.
Precast Agarose Gel Protocol
Prepare molten agarose gel solution using your standard protocol. Dilute ichorRed 10, 000X stock reagent into the molten agarose gel solution at 1:10, 000 (e.g. 5ul added to 50ml of the gel solution) and mix thoroughly. ichorRed can be added while the gel solution is still hot. Cast the gel and allow it to solidify. Any leftover gel solution may be stored and reheated later for additional gel casting. ichorRed precast gels may be stored at 4C for later use. Load samples and run the gels using your standard protocol. ichorRed: Image the stained gel with a standard transilluminator (302 or 312nm) use UV light box with EB filter.
Storage
Room temperature protected from light for at least one year
Use
Research Use Only

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5ml
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 02.05.2024 

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen