Vergleich

Rabbit antibody to USP13 (300-350)

ArtNr OSU00018W
Hersteller Osenses
Menge 100ul
Kategorie
Typ Antibody Polyclonal
Applikationen WB, IHC
Specific against Human (Homo sapiens)
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Ubiquitin carboxyl-terminal hydrolase 13, Deubiquitinating enzyme 13, Isopeptidase T-3, ISOT-3, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, ISOT3
Similar products ISOT3, Ubiquitin carboxyl-terminal hydrolase 13, Deubiquitinating enzyme 13, Isopeptidase T-3, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, ISOT-3
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Also known
Ubiquitin carboxyl-terminal hydrolase 13, Deubiquitinating enzyme 13, Isopeptidase T-3, ISOT-3, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, ISOT3
Target
Function: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
Tissue specificity: Highly expressed in ovary and testes.
Spcs X-react.
Human. Other species not yet tested but expected to work i mouse and rat.
Applications info
IHC, WB (confirmed by recombinant protein). Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Scortegagna M, et al. J. Biol. Chem. 286:27333-27341(2011)
Limitation
For research use only
Immunogen
A synthetic peptide from aa region 300-350 of human USP13 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen shares 93% identity with mouse and rat sequences.
Conjugate
Unconjugated antibody
Accession
http://www.uniprot.org/uniprot/Q92995
http://www.uniprot.org/uniprot/Q5BKP2
http://www.uniprot.org/uniprot/D3ZDI9
Purity
Whole serum
Clonality
Polyclonal
Specificity
Specific for USP13.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100ul
Lieferbar: In stock
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