Rabbit antibody to USP13 (300-350)

Function: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
Tissue specificity: Highly expressed in ovary and testes.

IHC, WB (confirmed by recombinant protein). Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Scortegagna M, et al. J. Biol. Chem. 286:27333-27341(2011)

A synthetic peptide from aa region 300-350 of human USP13 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen shares 93% identity with mouse and rat sequences.

http://www.uniprot.org/uniprot/Q92995
http://www.uniprot.org/uniprot/Q5BKP2
http://www.uniprot.org/uniprot/D3ZDI9

Polyclonal

Lyophilised