Rabbit antibody to HCN2

FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in neonatal and adult ventricle and in brain. Highly expressed in the pyramidal layer in hippocampus, in anterior dorsal nucleus in thalamus, in the mammillary nucleus in hypothalamus, in red nucleus, in trigeminal mesencephalic, spinal and principal nuclei, in cochlear and trapezoid nuclei and in the dorsal tegemental nucleus.
INDUCTION: By thyroid hormones in hypothyroid animals.
DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

IHC, WB. A dilution of 1 : 2000 is recommended for IHC and 1:500 for WB. The optimal dilution should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Kimura K, et al. Genes Cells 9:631-640(2004).

A synthetic peptide from mouse HCN2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.

http://www.uniprot.org/uniprot/Q9JKA9
http://www.uniprot.org/uniprot/O88703

Polyclonal

Lyophilised