Rabbit antibody to NG2

Valuable marker for several incompletely differentiated precursor cells.
Function: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Subcellular location: Apical cell membrane; Single-pass type I membrane protein; Extracellular side. Cell projection a€C lamellipodium membrane; Single-pass type I membrane protein; Extracellular side.
Tissue specificity: Expressed in microcascular pericytes and not endothelial cells. Neural cells and also extraneural tissues, especially in the developing mesenchyme.

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Nishiyama A, et al. J. Cell Biol. 114:359-371(1991)

A synthetic peptide from rat NG2 conjugated to blue carrier protein was used as the antigen.

http://www.uniprot.org/uniprot/Q00657

Polyclonal

Lyophilised