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CheKine™ Micro Hexokinase (HK) Activity Assay Kit

ArtNr ABK-KTB1123-96T
Hersteller Abbkine Scientific
Menge 96T
Kategorie
Typ Assay
Specific against other
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Alias Hexokinase;HK; Colorimetric Assay
Lieferbar
Applications Notes
CheKine™ Micro Hexokinase (HK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric HK detection method.HK catalyzes glucose synthesis of 6-phosphoglucose, and 6-phosphoglucose dehydrogenase further catalyzes 6-phosphoglucose dehydrogenation to NADH, which reduces WST-8 to orange formazan, with the maximum absorption peak detected at around 450 nm. The enzyme activity was calculated by detecting the increase rate of light absorption at 450 nm
Kit components
•Extraction Buffer
• Assay Buffer
• Substrate Mix
• Enzyme Mix
• WST-8
• Enhancer
• NADH Standard
Features & Benefits
• Detect hexokinase (HK) activity in bacterial supernatant of serum (plasma) animal and plant tissues/cells
• Detailed sample preparation and results calculation methods are provided
Calibration Range
31.25-2000μM
Usage Notes
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• It is advisable to measure serum potassium as soon as possible after blood collection.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage Instructions
Storage at -20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Hexokinase (HK) is widely found in animals, plants, microorganisms and cultured cells. It is the first key enzyme in the process of glucose decomposition, catalyzing the conversion of glucose to glucose 6-phosphoglucose, which is the intersection of glycolysis and pentose phosphate pathways
Booklet
KTB1123-B.pdf
Tag
HK
Description
CheKine™ Micro Hexokinase(HK) Assay Kit provides a simple and easy Colorimetric assay for measuring HK in samples.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96T
Lieferbar: In stock
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