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CheKine™ Micro Pyruvate Decarboxylase (PDC) Assay Kit

ArtNr ABK-KTB1125-96T
Hersteller Abbkine Scientific
Menge 96T
Kategorie
Typ Assay
Specific against other
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Alias oxynitrilase;pyruvate decarboxylase
Lieferbar
Applications Notes
The kit provides a simple method for detecting PDC activity in a variety of biological samples such as tissues, cell, serum, bacterial. The principle is that PDC catalyzes the decarboxylation of pyruvate to produce acetaldehyde, and alcohol dehydrogenase (ADH) is added to further catalyze the reduction of acetaldehyde by NADH to produce ethanol and NAD+. NADH has an absorption peak at 340 nm, but NAD+ does not. PDC activity is calculated by measuring the rate of decrease in light absorption at 340 nm.
Kit components
• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
Features & Benefits
• Determination of PDC activity in a variety of biological samples such as tissues, cell, serum, bacterial.
• Detailed sample preparation and result calculation methods are provided.
Usage Notes
• Extraction Buffer contains insoluble substance, shake well before use.
• If the change value of 1 min is small, the reaction time can be prolonged and the calculation formula time can be modified.
Storage Instructions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Pyruvate decarboxylase (PDC) is mainly found in yeast and is one of the key enzymes in ethanol fermentation. It catalyzes the decarboxylation of pyruvate to acetaldehyde. CheKineTM Pyruvate Decarboxylase (PDC) Activity Colorimetric Assay Kit provides a simple method for detecting PDC activity in animal and plant tissues, cells, bacteria, serum, plasma.
Booklet
KTB1125-B.pdf
Tag
PDC
Description
CheKine™ Micro Pyruvate Decarboxylase (PDC) Assay Kit is designed for detecting PDC activity in the sample.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96T
Lieferbar: In stock
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