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CheKine™ Micro Glutamic Acid Dehydrogenase(GDH)Assay Kit

ArtNr ABK-KTB3041-48T
Hersteller Abbkine Scientific
Menge 48T
Kategorie
Typ Assay
Specific against other
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Lieferbar
Applications Notes
The kit provides a simple method for detecting Glutamic Acid Dehydrogenase(GDH)activity in a variety of biological samples such as serum, plasma, tissues, cells , plants and bacteria.
Kit components
• ReagentⅠ
•ReagentⅡ
•ReagentⅢ
Features & Benefits
• Determination of GDH activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
Usage Notes
• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage Instructions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Glutamate (Glu) is widely found in animals, plants, microorganisms and cultured cells, and is one of the main sources of amino groups in organisms. Glutamate dehydrogenase (GDH) can catalyze the reversible oxidative dehydrogenation of glutamate to α-ketoglutarate, which is a key enzyme in carbon and nitrogen metabolism. GDH together with glutamate synthase (GOGAT), participates in the synthesis of glutamate and plays an important role in ammonia assimilation and conversion to organic nitrogen compounds.
Booklet
KTB3041-B.pdf
Tag
GDH
Description
CheKine™ Micro Glutamic Acid Dehydrogenase (GDH) Assay Kit provides a simple, convenient and rapid GDH activity detection method, which is suitable for the detection of animal and plant tissue, cell, bacteria, serum, plasma and other samples. The detection principle is that GDH catalyzes NH4 + , NADH and α-ketoglutaric acid to produce glutamic acid and NAD+ , which leads to the decrease of absorbance of 340 nm. By measuring the decreasing rate of absorbance of 340 nm, the activity of GDH is calculated.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 48T
Lieferbar: In stock
lieferbar

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