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Monkeypox Virus Protein Array G1

Monkeypox Virus Protein Array G1

ArtNr	PAV-MPHG-G1-32
Hersteller	Raybiotech
Menge	2 slides (32 wells)
Quantity options	1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells)
Kategorie	Arrays Protein Arrays
Typ	Array
Specific against	Human (Homo sapiens), Virus, Monkeypox
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	Arrays\|Disease Focused Arrays\|Protein Arrays
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Research Area	Mpox
Description	Monkeypox virus protein array is semi-quantitative array displaying 11 important Monkeypox virus proteins, such as A29L, A30L, A33R, A35R, B16R, C19L, D6L, E8L, H3L, I1L, L1R, etc. It is suitable for IgG, IgM, IgA, or any other isotype antibody detection.
Number of Targets Detected	11
Compatible Sample Types	Plasma, Serum, Ascites, Hybridoma cell culture medium, Other Body, Purified antibodies, Purified protein
Method of Detection	Fluorescence Laser Scanner
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Glass Slide
Kit Components	Ray Bio® monkeypox virus protein array Blocking Buffer Wash Buffer I Wash Buffer IIBiotin-conjugated secondary antibody Cy3 equivalent dye conjugated streptavidin Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)
Other Materials Required	Distilled water Small plastic boxes or containers Orbital shaker or oscillating rocker Pipettors, pipette tips and other common lab consumables Aluminum foil Laser fluorescence scanner View Compatible Laser Scanners Don't have a compatible scanner? Ray Biotech now offers F REE scanning service for all RayBio glass slide antibody arrays! Learn More
Product Features	Small sample volume required High density High sensitivity Large dynamic range Suitable for high-throughput assays
UNSPSC Code	41116133
Protein Name & Synonyms	A29L, A30L, A33R, A35R, B16R, C19L (identical to vaccinia virus F13L), D6L, E8L, H3L, I1L, L1R
Introduction	Recombinant Monkeypox virus (MPXV) proteins derived from bacterial and human cells (HEK293) are spotted in triplicate on a standard glass slide (25 mm x 75 mm x 1 mm), along with positive and negative controls to monitor each incubation step. When liquid sample types, such as serum or purified antibodies, are incubated on the array, "primary" antibodies in the sample will bind to their specific target proteins. Specific primary antibody isotypes (IgG, IgM, or IgA) from serum, plasma, purified antibodies, etc. are then targeted by biotin-conjugated anti-IgG, IgM, or IgA secondary antibodies, respectively, that are specific for the host species. A fluorophore-conjugated streptavidin molecule is then added, which binds to the biotin on the secondary antibody. The fluorophore enables the detection of the immobilized primary antibody via fluorescence using a laser scanner. The fluorescence signal is proportional to the amount of immobilized antibody. Since each spot represents a unique protein that is known, the specific target(s) bound by the antibodies can be ascertained.
Application Notes	Vaccine Development: Measure IgG, IgM, or IgA antibody responses to immunization or patients Protein-protein Interaction Studies: Determine the interactions between the viral proteins and other molecules, such as proteins, peptides, and small molecules. (Additional reagents and materials not included in the kit may be required.)
Number of Proteins Detected	11.0

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