Vergleich

Monkeypox Virus Protein Array G1

ArtNr PAV-MPHG-G1-32
Hersteller Raybiotech
Menge 2 slides (32 wells)
Quantity options 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells)
Kategorie
Typ Array
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rabbit (Oryctolagus cuniculus)
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Category
Arrays|Disease Focused Arrays|Protein Arrays
Shipping Temperature
Blue ice
Storage Conditions
-20°C
Compatible Sample Types
Ascites
Hybridoma cell culture medium
Other fluids
Plasma
Purified antibodies
Purified protein
Serum
Method of Detection
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
Glass Slide
Kit Components
Ray Bio® monkeypox virus protein array
Blocking Buffer
Wash Buffer I
Wash Buffer IIBiotin-conjugated secondary antibody
Cy3 equivalent dye conjugated streptavidin
Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)
Other Materials Required
Distilled water
Small plastic boxes or containers
Orbital shaker or oscillating rocker
Pipettors, pipette tips and other common lab consumables
Aluminum foil
Laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? Ray Biotech now offers F
REE scanning service for all RayBio glass slide antibody arrays! Learn More
Product Features
Small sample volume required
High density
High sensitivity
Large dynamic range
Suitable for high-throughput assays
UNSPSC Code
41116133
Protein Name & Synonyms
A29L, A30L, A33R, A35R, B16R, C19L (identical to vaccinia virus F13L), D6L, E8L, H3L, I1L, L1R
Introduction
Recombinant Monkeypox virus (MPXV) proteins derived from bacterial and human cells (HEK293) are spotted in triplicate on a standard glass slide (25 mm x 75 mm x 1 mm), along with positive and negative controls to monitor each incubation step. When liquid sample types, such as serum or purified antibodies, are incubated on the array, "primary" antibodies in the sample will bind to their specific target proteins. Specific primary antibody isotypes (IgG, IgM, or IgA) from serum, plasma, purified antibodies, etc. are then targeted by biotin-conjugated anti-IgG, IgM, or IgA secondary antibodies, respectively, that are specific for the host species.
A fluorophore-conjugated streptavidin molecule is then added, which binds to the biotin on the secondary antibody. The fluorophore enables the detection of the immobilized primary antibody via fluorescence using a laser scanner. The fluorescence signal is proportional to the amount of immobilized antibody. Since each spot represents a unique protein that is known, the specific target(s) bound by the antibodies can be ascertained.
Application Notes
Vaccine Development: Measure IgG, IgM, or IgA antibody responses to immunization or patients
Protein-protein Interaction Studies: Determine the interactions between the viral proteins and other molecules, such as proteins, peptides, and small molecules. (Additional reagents and materials not included in the kit may be required.)
Number of Proteins Detected
11.0

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 2 slides (32 wells)
Lieferbar: In stock
lieferbar

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