Uracil-DNA Glycosylase(UNG), Heat-labile

An enzyme that is used to prevent PCR carry-over and false positives

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Uracil-DNA Glycosylase (UNG) hydrolyzes the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an apyrimidinic site in DNA. Since DNA with an apyrimidinic site is degraded by heat trea™ent, UNG can be used in combination with dUTP to prevent carry-over of amplified products derived from PCR.This enzyme hydrolyzes uracil from both single- and double-stranded DNA containing dU, but it not from RNA. It can also be used to prevent carryover of RT-PCR.We also have a glycerol-free version that is suitable for lyophilization.