GULP1, Recombinant, Human, aa1-304, His-Tag (PTB Domain-containing Engulfment Adapter Protein 1, CED-6, CED6, GULP)

Blue Ice

36, 9

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 50% glycerol, 2mM DTT.

PTB domain-containing engulfment adapter protein 1, also known as GULP1, is an evolutionarily conserved adaptor protein required for efficient engulfment of apoptotic cells by phagocytes. GULP1 also helps modulate cellular glycosphingolipid and cholesterol transport. It also may play a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increased cytoplasmic levels of GULP1 are associated with increases in cellular levels of GTP-bound ARF6.

Source:
Recombinant protein corresponding to human GULP1, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~36.9kD (327aa) (MALDI-TOF)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMNRAFSR KKDKTWMHTP EALSKHFIPY NAKFLGSTEV EQPKGTEVVR DAVRKLKFAR HIKKSEGQKI PKVELQISIY GVKILEPKTK EVQHNCQLHR ISFCADDKTD KRIFTFICKD SESNKHLCYV FDSEKCAEEI TLTIGQAFDL AYRKFLESGG KDVETRKQIA GLQKRIQDLE TENMELKNKV QDLENQLRIT QVSAPPAGSM TPKSPSTDIF DMIPFSPISH QSSMPTRNGT QPPPVPSRST EIKRDLFGAE PFDPFNCGAA DFPPDIQSKL DEMQEGFKMG LTLEGTVFCL DPLDSRC

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.