MOB1B, Recombinant, Human, 1-216aa, His-Tag (MOB Kinase Activator 1B Isoform 2, MATS2, MOB4A, MOBKL1A)

Blue Ice

27

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 30% glycerol, 1mM DTT.

MOB1B is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Three transcript variants encoding different isoforms have been found for this gene.

Source:
Recombinant protein corresponding to human MOB1B, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~27kD (239aa) (MALDI-TOF)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMSFLFGS RSSKTFKPKK NIPEGSHQYE LLKHAEATLG SGNLRMAVML PEGEDLNEWV AVNTVDFFNQ INMLYGTITD FCTEESCPVM SAGPKYEYHW ADGTNIKKPI KCSAPKYIDY LMTWVQDQLD DETLFPSKIG VPFPKNFMSV AKTILKRLFR VYAHIYHQHF DPVIQLQEEA HLNTSFKHFI FFVQEFNLID RRELAPLQEL IEKLTSKDR

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.