NDUFB4, Recombinant, Human, aa1-87, His-Tag (NADH Dehydrogenase 1 Beta Subcomplex Subunit 4, B15, CI-B15)

Blue Ice

12, 6

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.

NDUFB4 is a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.

Source:
Recombinant protein corresponding to human NDUFB4, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~12.6kD (110aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMSFPKYK PSSLRTLPET LDPAEYNISP ETRRAQAERL AIRAQLKREY LLQYNDPNRR GLIENPALLR WAYARTINVY PNFRPTPKNS

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.